Ouyang Q, Vilien M, Juhl B R, Larsen L G, Binder V
Acta Pathol Microbiol Immunol Scand A. 1987 Jul;95(4):177-83. doi: 10.1111/j.1699-0463.1987.tb00028_95a.x.
Antibodies raised against tumour-associated antigens have been assessed for tumour selectivity using an indirect immunohistochemical peroxidase staining of formalin-fixed, paraffin-embedded tissue from colon carcinomas, colon polyps and normal mucosa. The following antibodies were used: 1) Unabsorbed polyclonal antibody to carcinoembryonic antigen (poly-CEA). 2) Monoclonal antibodies to CEA (mabs 3851 and 27). 3) Monoclonal antibodies to protein-bound carbohydrates (mabs C 216 and C 242) or to lipid- and protein-bound carbohydrates (C 50 and 19-9). These antibodies had been produced by hybridization of lymphocytes from mice, immunized with colon carcinoma cell lines or colon cancer tissue. All antibodies were used in one concentration only, preselected by initial titration experiments. No antibody was completely tumour-specific, but four antibodies, mabs 3851, 27, C 216 and C 242, showed statistically significant tumour selectivity. Using these antibodies, respectively 19, 19, 19, and 18 of 20 colon cancer were stained compared with 3, 4, 4, and 8 of 15 specimens of colon mucosa from normal controls. An increased frequency of staining was also noted in dysplastic polyps (statistically significant using mabs 3851 and C 216) and in dysplastic mucosa adjacent to a tumour (statistically significant using mabs 3851 and 27). The staining frequency of normal colon mucosa in cases of colon cancer did not differ from that in the normal controls. Poly-CEA and the anti-ganglioside mabs C 50 and 19-9 revealed no tumour selectivity. A pronounced goblet cell staining was seen using C 50, C 242 and 19-9.
通过对来自结肠癌、结肠息肉和正常黏膜的福尔马林固定、石蜡包埋组织进行间接免疫组织化学过氧化物酶染色，评估了针对肿瘤相关抗原产生的抗体的肿瘤选择性。使用了以下抗体：1）未吸收的癌胚抗原多克隆抗体（多克隆CEA）。2）CEA单克隆抗体（单克隆抗体3851和27）。3）针对蛋白结合碳水化合物的单克隆抗体（单克隆抗体C 216和C 242）或针对脂质和蛋白结合碳水化合物的单克隆抗体（C 50和19-9）。这些抗体是通过用结肠癌细胞系或结肠癌组织免疫的小鼠淋巴细胞杂交产生的。所有抗体仅以一种浓度使用，该浓度通过初始滴定实验预先选定。没有一种抗体是完全肿瘤特异性的，但四种抗体，即单克隆抗体3851、27、C 216和C 242，显示出具有统计学意义的肿瘤选择性。使用这些抗体，20例结肠癌中分别有19、19、19和18例被染色，而正常对照的15例结肠黏膜标本中分别有3、4、4和8例被染色。在发育异常的息肉中（使用单克隆抗体3851和C 216具有统计学意义）以及肿瘤旁发育异常的黏膜中（使用单克隆抗体3851和27具有统计学意义）也观察到染色频率增加。结肠癌病例中正常结肠黏膜的染色频率与正常对照无差异。多克隆CEA以及抗神经节苷脂单克隆抗体C 50和19-９未显示出肿瘤选择性。使用C 50、C 242和19-９可见明显的杯状细胞染色。
