Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Department of Periodontal Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
J Oral Biosci. 2020 Dec;62(4):322-326. doi: 10.1016/j.job.2020.09.007. Epub 2020 Oct 8.
The periodontal pathogen Porphyromonas gingivalis produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in the organism's virulence, and pigmentation. We previously showed that deletion of the PGN_0297 and PGN_0300 genes reduced the proteolytic activity of gingipains. The role of the PGN_0296 gene, consisting of an operon with the PGN_0297 and PGN_0300 genes, is unclear. Herein, we examined the effect of PGN_0296 gene deletion on the proteolytic activity. Although the proteolytic activity of the gingipains did not decrease in the culture supernatant of a PGN_0296 gene deletion mutant (ΔPGN_0296), the growth was delayed.
牙周病原体牙龈卟啉单胞菌产生牙龈蛋白酶（Kgp、RgpA 和 RgpB），这是一种与生物体毒力和色素沉着有关的半胱氨酸蛋白酶。我们之前的研究表明，缺失 PGN_0297 和 PGN_0300 基因可降低牙龈蛋白酶的蛋白水解活性。PGN_0296 基因由与 PGN_0297 和 PGN_0300 基因组成的操纵子组成，其作用尚不清楚。在此，我们研究了 PGN_0296 基因缺失对蛋白水解活性的影响。尽管 PGN_0296 基因缺失突变体（ΔPGN_0296）的培养上清液中的牙龈蛋白酶活性没有降低，但生长延迟。
