Cornette J C, Evans D B, Furlong A M, Sharma S K
Anal Biochem. 1987 May 15;163(1):93-9. doi: 10.1016/0003-2697(87)90097-2.
A method for human renin activity determination using human plasma as the substrate is described. Angiotensin I is generated by incubating renin with human plasma, which is available along with the commercial radioimmunoassay kit for angiotensin I. The method obviates the need to isolate and purify the substrate, human angiotensinogen, from human plasma. In addition, the assay is highly renin specific, sensitive, and convenient to use for the routine determination of active human renin during its isolation and purification from tissue extracts or from genetically engineered bacterial and nonbacterial expression systems.
描述了一种以人血浆为底物测定人肾素活性的方法。肾素与人血浆孵育可生成血管紧张素I，人血浆可与血管紧张素I商业放射免疫分析试剂盒一起获得。该方法无需从人血浆中分离和纯化底物人血管紧张素原。此外，该测定对肾素具有高度特异性、敏感性，并且便于在从组织提取物或基因工程细菌及非细菌表达系统中分离和纯化活性人肾素的过程中进行常规测定。
