Tsubouchi S, Kano E, Suzuki H
Anat Rec. 1987 Jun;218(2):111-5. doi: 10.1002/ar.1092180203.
The acinar and islet cells of the adult mouse pancreas were examined by radioautography after continuous infusion of 3H-thymidine, for periods varying from 1 to 60 days, to determine whether they behaved like renewing or expanding cell populations. The labeling of both cell types increased with the duration of the continuous infusion and reached 2.22% and 12.0%, respectively, after 60 days. The rate of acinar and islet cell labeling was estimated from the regression line of the labeling index versus time and given as 0.039% and 0.20% cells per day, respectively. The rate of cell labeling was relatively low in these acinar and islet cells in comparison to the relatively high rate in duct cells. Occasionally, acinar cell labeling was not uniform, showing high labeling in the outer peripheral region of a lobe and at the periphery of the islets. Both acinar and islet cells increased in number in the adult, and at a rate indicating they are expanding cell populations. Their doubling times were estimated as 2,564 days (7.0 years) and 500 days (1.3 years), respectively. Duct epithelial cell populations were dividing at a rate indicating that they are renewing cell populations.
在连续输注³H-胸腺嘧啶核苷1至60天不等的时间后，通过放射自显影术对成年小鼠胰腺的腺泡细胞和胰岛细胞进行了检查，以确定它们的行为是否类似于更新或增殖的细胞群体。两种细胞类型的标记率均随连续输注时间的延长而增加，60天后分别达到2.22%和12.0%。根据标记指数与时间的回归线估算腺泡细胞和胰岛细胞的标记率，分别为每天0.039%和0.20%的细胞。与导管细胞相对较高的标记率相比，这些腺泡细胞和胰岛细胞的细胞标记率相对较低。偶尔，腺泡细胞标记并不均匀，在叶的外周区域和胰岛的周边显示出高标记。成年小鼠的腺泡细胞和胰岛细胞数量均增加，且其增加速率表明它们是增殖性细胞群体。它们的倍增时间分别估计为2564天（7.0年）和500天（1.3年）。导管上皮细胞群体的分裂速率表明它们是更新性细胞群体。
