Ascorbic acid: the concept of optimum requirements.

Experiments with enzymes in situ that are dependent on ascorbic acid for maximum activity will provide critical information about ascorbic acid requirements. Our work with chromaffin tissue as a model system eventually will result in the determination of two dose-response curves for norepinephrine biosynthesis, representing cytosolic ascorbic acid and intragranular ascorbic acid (Fig. 11). These curves for norepinephrine biosynthesis can be combined with curves for other enzymatic events that are also dependent on ascorbic acid for maximal activity. These dose-response curves (Fig. 2) will allow determination of optimum ascorbic acid requirements based on specific product formation and minimum toxicity. These principles are adaptable to other vitamins as well as ascorbic acid, and could form the basis for a new approach to vitamin requirements.