miR-381-3p/RAB2A 轴激活膀胱癌细胞增殖并抑制细胞凋亡。

MiR-381-3p/RAB2A axis activates cell proliferation and inhibits cell apoptosis in bladder cancer.

机构信息

Department of Urology, The Third Hospital of Shandong Province, Jinan, 250031, China.

Department of Internal Medicine, The People Hospital of Huaiyin, jinan, 250021,China.

出版信息

Cell Mol Biol (Noisy-le-grand). 2020 Sep 30;66(6):117-120.

Abstract

Acting as a really common cancer in the world, bladder cancer has taken many people's life away. MiRNAs and mRNA have been reported can regulate the expression of cancers. In this study, the role of RAB2A and miR-381-3p was fully studied in bladder cancer. qRT-PCR assay probe the expression of RAB2A and miR-381-3p in bladder cancer cells. Meanwhile, colony formation assay, EdU assay, flow cytometry analysis, JC-1 assay and western blot assay were implemented to detect the progression of bladder cancer cells. Silenced RAB2A could reduce the cell proliferation of bladder cancer, and activate the apoptosis. Meanwhile, miR-381-3p could bind to RAB2A in bladder cancer cells and overexpressed miR-381-3p could inhibit the progression of bladder cancer cells. MiR-381-3p/RAB2A axis activates cell proliferation and inhibits cell apoptosis in bladder cancer.

摘要

作为世界上一种常见的癌症，膀胱癌已经夺走了许多人的生命。miRNAs 和 mRNA 已被报道可以调节癌症的表达。在这项研究中，RAB2A 和 miR-381-3p 在膀胱癌中的作用得到了充分研究。qRT-PCR 检测膀胱癌细胞中 RAB2A 和 miR-381-3p 的表达。同时，进行集落形成试验、EdU 试验、流式细胞术分析、JC-1 试验和 Western blot 试验检测膀胱癌细胞的进展。沉默 RAB2A 可减少膀胱癌细胞的增殖，并激活细胞凋亡。同时，miR-381-3p 可与膀胱癌细胞中的 RAB2A 结合，过表达 miR-381-3p 可抑制膀胱癌细胞的进展。miR-381-3p/RAB2A 轴激活膀胱癌细胞增殖，抑制细胞凋亡。