Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Cell Mol Biol (Noisy-le-grand). 2020 Sep 30;66(6):121-126.
Urtica dioica agglutinin (UDA) is a very small plant lectin with anti-prostatic activity. In this study, we investigated the effect of UDA on proliferation and apoptosis induction in human acute lymphoid leukemia (ALL) cell lines. The effect of UDA on Jurkat and Raji cell proliferation was examined by MTS assay. Distribution of cell cycle phases was determined by PI staining and apoptosis was examined with annexin V/PI and western blot. Results showed UDA treatment reduced cell proliferation in cells by inducing apoptosis. PI staining was associated with a higher percentage of the cell population in sub G1. Caspase-8 and caspase-9 dependent apoptosis occurred in Jurkat cells. Generally, UDA treatment resulted in cell death in ALL cell lines and induced apoptosis in the T-ALL cell line, Jurkat, through extrinsic and intrinsic pathways. These results may be considered as a guide to working on UDA as an anti-leukemic drug in the future.
荨麻凝集素 (UDA) 是一种具有抗前列腺活性的非常小的植物凝集素。在这项研究中，我们研究了 UDA 对人急性淋巴细胞白血病 (ALL) 细胞系增殖和凋亡诱导的影响。通过 MTS 测定法检查 UDA 对 Jurkat 和 Raji 细胞增殖的影响。通过 PI 染色确定细胞周期各阶段的分布，并用 annexin V/PI 和 Western blot 检测细胞凋亡。结果表明，UDA 通过诱导细胞凋亡来减少细胞增殖。PI 染色与亚 G1 期细胞群体的比例更高相关。Jurkat 细胞中发生了 caspase-8 和 caspase-9 依赖性凋亡。一般来说，UDA 处理导致 ALL 细胞系中的细胞死亡，并通过外在和内在途径诱导 T-ALL 细胞系 Jurkat 细胞凋亡。这些结果可被视为未来将 UDA 作为抗白血病药物进行研究的指导。
