从酿酒酵母中分离出的多核糖体和可溶性因子对5'-磷酸7-甲基鸟苷抑制mRNA翻译的逆转作用。
Reversion of 7-methylguanosine 5'-phosphate inhibition of mRNA translation by polysomal and soluble factors isolated from Saccharomyces cerevisiae.
作者信息
Parets Suler A, Zueco J, Gozalbo D, Sentandreu R
出版信息
Biochem Biophys Res Commun. 1987 Aug 14;146(3):1093-101. doi: 10.1016/0006-291x(87)90760-1.
Protein fractions that overcome m7GMP inhibition of mRNA translation have been purified from the yeast S. cerevisiae. An active fraction isolated from polysomes contains two polypeptides of 220- and 190-kDa. The active fraction isolated from postribosomal supernatant contains a major polypeptide of 28-kDa and other species of 32-, 24-, 22- and 21-kDa, and sediments in sucrose gradients as a high molecular weight complex of about 200,000. This fraction restored yeast mRNA translation in reticulocyte lysates under conditions of yeast and globin mRNA competition; however, this effect was not observed with the 220- and 190-kDa polypeptides from polysomes. Nevertheless, translation of yeast mRNA was stimulated by a partially purified fraction containing a 28-kDa polypeptide from polysomes.
已从酿酒酵母中纯化出能克服m7GMP对mRNA翻译抑制作用的蛋白质组分。从多核糖体中分离出的活性组分含有两条分别为220 kDa和190 kDa的多肽。从核糖体后上清液中分离出的活性组分含有一条主要的28 kDa多肽以及32 kDa、24 kDa、22 kDa和21 kDa的其他组分，并在蔗糖梯度中沉降为约200,000的高分子量复合物。在酵母mRNA和珠蛋白mRNA竞争的条件下，该组分可恢复网织红细胞裂解物中酵母mRNA的翻译；然而，来自多核糖体的220 kDa和190 kDa多肽未观察到这种效应。尽管如此，含有来自多核糖体的28 kDa多肽的部分纯化组分可刺激酵母mRNA的翻译。
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