Doctoral School of Health Sciences, Faculty of Health Science, University of Pécs, Pécs, Hungary.
Department of Medicine and Nephrological Center, Medical School, University of Pécs, Pécs, Hungary.
J Clin Lab Anal. 2021 Feb;35(2):e23613. doi: 10.1002/jcla.23613. Epub 2020 Oct 11.
Under conditions of oxidative stress, hydroxyl radicals can oxidize phenylalanine (Phe) into various tyrosine (Tyr) isomers (meta-, ortho-, and para-tyrosine; m-, o-, and p-Tyr), depending on the location of the hydroxyl group on the oxidized benzyl ring. This study aimed to compare patients with ST-segment elevation myocardial infarction (STEMI) and non-STEMI (NSTEMI) and the serum levels of Phe and Tyr isomers at the aortic root and distal to the culprit lesion in both groups.
Forty-four patients participated in the study: 23 with STEMI and 21 with NSTEMI. Arterial blood samples were taken from the aortic root through a guiding catheter and from the culprit vessel segment distal from the primary lesion with an aspiration catheter, during the percutaneous coronary intervention. Serum levels of Phe, p-Tyr, m-Tyr, and o-Tyr were determined using reverse-phase high-performance liquid chromatography.
Serum levels of Phe were significantly higher distal to the culprit lesion compared to the aortic root in patients with STEMI. Serum p-Tyr/Phe and m-Tyr/Phe concentration ratios were both lower distal to the culprit lesion than at the aortic root in patients with STEMI. There were no statistically significant differences with respect to changes in serum Phe and Tyr isomers distal to the culprit lesion compared to the aortic root in patients with NSTEMI.
Our data suggest that changes in serum levels of different Tyr isomers can mediate the effects of oxidative stress during myocardial infarction.
在氧化应激条件下，羟自由基可以将苯丙氨酸（Phe）氧化成各种酪氨酸（Tyr）异构体（间位、对位和邻位酪氨酸；m-、o-和 p-Tyr），具体取决于氧化苄基环上羟基的位置。本研究旨在比较 ST 段抬高型心肌梗死（STEMI）和非 ST 段抬高型心肌梗死（NSTEMI）患者以及两组患者主动脉根部和罪犯病变远端血清 Phe 和 Tyr 异构体水平。
44 名患者参与了这项研究：23 名 STEMI 患者和 21 名 NSTEMI 患者。在经皮冠状动脉介入治疗过程中，通过引导导管从主动脉根部和抽吸导管从主要病变远端的罪犯血管段采集动脉血样。采用反相高效液相色谱法测定血清中 Phe、p-Tyr、m-Tyr 和 o-Tyr 的水平。
STEMI 患者罪犯病变远端血清 Phe 水平明显高于主动脉根部。STEMI 患者罪犯病变远端血清 p-Tyr/Phe 和 m-Tyr/Phe 浓度比值均低于主动脉根部。NSTEMI 患者罪犯病变远端与主动脉根部相比，血清 Phe 和 Tyr 异构体无统计学差异。
我们的数据表明，不同 Tyr 异构体血清水平的变化可以介导心肌梗死后氧化应激的影响。
