Resistance of attached Escherichia coli to acrylic acid and its significance for the survival of plasmid-bearing organisms in water.

As previously reported, free organisms of Escherichia coli are sensitive to damage and killing when exposed to acrylic acid in water. The effect of the agent was greatest in distilled water, but there was a marked effect in effluent and seawater also. The effect was temperature-dependent, with organisms exposed at 4 degrees C being much less affected than those exposed at 20 degrees C. The above sensitivity was for free organisms, but those attached to glass beads were resistant to acrylate. This resistance applied equally to attached plasmid-free and attached plasmid-bearing organisms, but is likely to be more significant for plasmid-bearing strains because some plasmids studied here stimulated bacterial attachment. The likely significance of the acrylate resistance of attached organisms for enterobacterial survival in the aquatic environment, e.g. in the vicinity of shellfish beds, is discussed.