Maruno M, Yoshimine T, Ushio Y, Hayakawa T, Jamshidi J, Arita N, Bitoh S, Mogami H
No To Shinkei. 1987 Jun;39(6):579-85.
Distributions of two different subclasses of intermediate filaments, vimentin and glial filaments, were studied immunohistochemically in human brain tumors using specific antiserum to each protein subunit, vimentin and astroprotein (GFAP), Surgical specimens (5 meningiomas, 4 ependymomas, 5 benign astrocytomas, 5 anaplastic astrocytomas and 7 glioblastomas) were fixed in 95% ethanol or ethanol-acidic acid (95:5) and embedded in paraffin Avidin biotin peroxidase-complex (ABC) method (Vectastain) was carried out on 6 microns-thick paraffin sections. All meningioma cells were negative for astroprotein (GFAP) and positive for vimentin. Ependymoma cells showed various patterns of immunoreaction for astroprotein (GFAP) but were invariably positive for vimentin. In benign astrocytomas, many cells (or cell body and processes) were positive for astroprotein (GFAP). Immunoreaction for vimentin was, however, less frequent and intense. In anaplastic astrocytomas, population of astroprotein (GFAP)-positive cells decreased and vimentin-positive cells increased. Astroprotein (GFAP)-positive cells were further decreased in glioblastomas and the population of vimentin-positive cells varied among tissues. The present study suggests that the anaplastic change of astrocytoma cells were associated with decreased expression of glial filaments and increased expression of vimentin filaments. It was also suggests that the expression of both intermediate filaments may be suppressed in highly-malignant glial tumor cells.
使用针对波形蛋白和星形胶质蛋白（胶质纤维酸性蛋白，GFAP）各蛋白亚基的特异性抗血清，通过免疫组织化学方法研究了两种不同亚类中间丝（波形蛋白和神经胶质丝）在人脑肿瘤中的分布。手术标本（5例脑膜瘤、4例室管膜瘤、5例良性星形细胞瘤、5例间变性星形细胞瘤和7例胶质母细胞瘤）用95%乙醇或乙醇 - 乙酸（95:5）固定，然后石蜡包埋。对6微米厚的石蜡切片采用抗生物素蛋白 - 生物素过氧化物酶复合物（ABC）法（Vectorstain）。所有脑膜瘤细胞对星形胶质蛋白（GFAP）呈阴性，对波形蛋白呈阳性。室管膜瘤细胞对星形胶质蛋白（GFAP）呈现出不同的免疫反应模式，但对波形蛋白始终呈阳性。在良性星形细胞瘤中，许多细胞（或细胞体及突起）对星形胶质蛋白（GFAP）呈阳性。然而，波形蛋白的免疫反应频率较低且强度较弱。在间变性星形细胞瘤中，星形胶质蛋白（GFAP）阳性细胞数量减少，波形蛋白阳性细胞数量增加。在胶质母细胞瘤中，星形胶质蛋白（GFAP）阳性细胞进一步减少，波形蛋白阳性细胞数量在不同组织中有所不同。本研究表明，星形细胞瘤细胞的间变改变与神经胶质丝表达减少和波形蛋白丝表达增加有关。还表明，在高度恶性的胶质肿瘤细胞中，两种中间丝的表达可能均受到抑制。
