Hochman-Mendez Camila, Coelho-Sampaio Tatiana, Kent Ariel J, Inman Jamie L, Bissell Mina J, Robertson Claire
Regenerative Medicine Research, Texas Heart Institute.
Institute of Biomedical Sciences, Federal University of Rio de Janeiro.
J Vis Exp. 2020 Sep 28(163). doi: 10.3791/61134.
Laminin-111 (Ln1) is an essential part of the extracellular matrix in epithelia, muscle and neural systems. We have previously demonstrated that the microstructure of Ln1 alters the way that it signals to cells, possibly because Ln1 assembly into networks exposes different adhesive domains. In this protocol, we describe three methods to generate polymerized Ln1.
层粘连蛋白-111(Ln1)是上皮、肌肉和神经系统细胞外基质的重要组成部分。我们之前已经证明,Ln1的微观结构会改变其向细胞发出信号的方式,这可能是因为Ln1组装成网络会暴露出不同的黏附结构域。在本实验方案中,我们描述了三种生成聚合Ln1的方法。