Turski W A, Herrling P L, Do K Q
Brain Res. 1987 Jun 30;414(2):330-8. doi: 10.1016/0006-8993(87)90014-x.
Responses evoked by L-cysteine-sulphinate (L-CSA) and L-aspartate (L-Asp) were recorded with intracellular electrodes from caudate neurons in halothane anesthetized cats. L-CSA and L-Asp were applied microiontophoretically to caudate cells and their effects on membrane and action potentials, as well as on cortically evoked synaptic potentials were evaluated. L-CSA and L-Asp induced depolarizations accompanied by regular firing resembling kainate (KA)- or quisqualate (QUIS)-induced excitation patterns (type 1) in 82% and 72% of the recorded neurons, respectively, and a mixed pattern consisting of a N-methyl-D-aspartate (NMDA)-like excitation (type 2) followed by a regular type 1 pattern in the remaining cells. In about a quarter of the cells the effects of L-CSA and L-Asp, but not those of KA or QUIS, were partially antagonized by 2-amino-7-phosphonoheptanoate (AP-7), a specific NMDA receptor antagonist. Kynurenate, a broad spectrum excitatory amino acid antagonist, blocked responses elicited by either L-CSA or QUIS. The actions of L-CSA and L-Asp on the firing pattern and membrane potential of cat caudate neurons in situ provide evidence in favor of their mixed agonist nature with respect to NMDA and non-NMDA excitatory amino acid receptors.
在氟烷麻醉的猫中，用细胞内电极记录了尾状核神经元对L-半胱氨酸亚磺酸盐（L-CSA）和L-天冬氨酸（L-Asp）的反应。将L-CSA和L-Asp通过微离子电泳法施加于尾状核细胞，并评估它们对膜电位和动作电位以及皮层诱发的突触电位的影响。L-CSA和L-Asp分别在82%和72%的记录神经元中诱导去极化，并伴有类似于 kainate（KA）或quisqualate（QUIS）诱导的兴奋模式（1型）的规则放电，其余细胞则呈现由N-甲基-D-天冬氨酸（NMDA）样兴奋（2型）后接规则1型模式组成的混合模式。在大约四分之一的细胞中，L-CSA和L-Asp的作用，但不是KA或QUIS的作用，被特异性NMDA受体拮抗剂2-氨基-7-磷酸庚酸（AP-7）部分拮抗。犬尿氨酸是一种广谱兴奋性氨基酸拮抗剂，可阻断L-CSA或QUIS引发的反应。L-CSA和L-Asp对猫原位尾状核神经元放电模式和膜电位的作用提供了证据，支持它们对NMDA和非NMDA兴奋性氨基酸受体具有混合激动剂性质。
