Evidence for a glycinergic pathway connecting the two cochlear nuclei: an immunocytochemical and retrograde transport study.

A combined retrograde transport and immunocytochemical study was carried out to determine the projections of the large glycine-immunoreactive neurons in the ventral cochlear nucleus of the guinea pig. Horseradish peroxidase (HRP) was injected into the contralateral cochlear nucleus, and cochlear nucleus sections were developed for HRP activity followed by staining for glycine using affinity-purified anti-glycine antibodies. These studies show that glycine-immunoreactive neurons in the ventral cochlear nucleus project to the contralateral cochlear nucleus and suggest that this pathway may be glycinergic.