St. Petersburg University, 7/9 Universitetskaya Emb., St. Petersburg, 199034, Russia.
Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy Prospect V.O. 31, St. Petersburg, 199004, Russia.
Nanotechnology. 2021 Jan 22;32(4):045604. doi: 10.1088/1361-6528/abc037.
Nanostructures synthesized using DNA-conjugated gold nanoparticles have a wide range of applications in the field of biosensorics. The stability of the DNA duplex plays a critical role as it determines the final geometry of these nanostructures. The main way to control DNA stability is to maintain a high ionic strength of the buffer solution; at the same time, high salt concentrations lead to an aggregation of nanoparticles. In this study, by means of the instrumentality of DNA-bridged seeds using tris(hydroxymethyl)aminomethane as a soft reducing agent the dumbbell-like gold nanoparticles up to 35 nm were synthesized with a high concentration of sodium ions of up to 100 mM and magnesium ions up to 1 mM. We also examined at the atomic level the details of the effect of the gold nanoparticle surface, as well as Na and Mg ions, on the stability of nucleotide pairs located in close proximity to the grafting site.
使用 DNA 修饰的金纳米粒子合成的纳米结构在生物传感器领域有广泛的应用。DNA 双链的稳定性起着关键作用，因为它决定了这些纳米结构的最终几何形状。控制 DNA 稳定性的主要方法是保持缓冲溶液的高离子强度；同时，高盐浓度会导致纳米粒子聚集。在这项研究中，通过使用三羟甲基氨基甲烷作为软还原剂的 DNA 桥接种子的手段，在高达 100mM 的钠离子和高达 1mM 的镁离子的高浓度下合成了长达 35nm 的哑铃状金纳米粒子。我们还在原子水平上研究了金纳米粒子表面以及 Na 和 Mg 离子对靠近嫁接位点的核苷酸对稳定性的影响细节。
