培养的成纤维细胞对人胰岛素、牛胰岛素和猪胰岛素的敏感性。

Sensitivity of cultured fibroblasts to human, bovine and porcine insulins.

作者信息

Hernvann A, Cynober L, Aussel C, Agneray J, Ekindjian O

出版信息

Cell Biol Int Rep. 1987 Aug;11(8):591-8. doi: 10.1016/0309-1651(87)90139-1.

Abstract

We have studied the effects of human, bovine and porcine insulin on sugar transport by cultured chicken embryo fibroblast monolayers. For a 30 min. association time, human and bovine insulin at a concentration of 5.10(-8) M stimulated 2-deoxy-D-glucose uptake. (respectively by an average 58 p.cent and 55 p.cent over basal). Porcine insulin was less potent since a concentration of 5.10(-7) M was necessary to obtain similar stimulation. Moreover, the maximal effect of porcine insulin occur only after 60 min. association time instead of 30 min. for the other peptides. The differences between the effects of insulin from different sources is related to species-dependent differences in their structure.

摘要

我们研究了人胰岛素、牛胰岛素和猪胰岛素对培养的鸡胚成纤维细胞单层糖转运的影响。在30分钟的结合时间内，浓度为5×10⁻⁸ M的人胰岛素和牛胰岛素刺激了2-脱氧-D-葡萄糖的摄取（分别比基础水平平均高58%和55%）。猪胰岛素的效力较低，因为需要5×10⁻⁷ M的浓度才能获得类似的刺激。此外，猪胰岛素的最大效应仅在60分钟的结合时间后出现，而其他肽在30分钟后出现。不同来源胰岛素作用的差异与它们结构上的物种依赖性差异有关。