Papamatheakis J D, Marciani D J
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2784-8. doi: 10.1073/pnas.76.6.2784.
Addition of purified major glycoprotein from avian myeloblastosis virus to growing or quiescent chicken embryo fibroblasts rapidly stimulates the rate of hexose transport and increases the lactic acid production. These stimulatory effects are dependent on the time of exposure and the dose of viral glycoprotein. In contrast, the glycoprotein only marginally affects hexose transport in chicken cells transformed by Rous sarcoma virus. Some effects of the glycoprotein on serum-starved quiescent cells were similar to those observed upon re-addition of serum; however, the viral glycoprotein did not stimulate DNA synthesis. Quiescent cells stimulated by saturating levels of serum showed little further stimulation of hexose uptake upon exposure to viral glycoprotein for 3 hr. This behavior suggests that the glycoprotein may be acting on a system that is also a target for serum action.
向生长中的或静止的鸡胚成纤维细胞中添加来自禽成髓细胞瘤病毒的纯化主要糖蛋白，可迅速刺激己糖转运速率并增加乳酸生成。这些刺激作用取决于暴露时间和病毒糖蛋白的剂量。相比之下，该糖蛋白对劳氏肉瘤病毒转化的鸡细胞中的己糖转运仅有轻微影响。该糖蛋白对血清饥饿的静止细胞的一些作用与重新添加血清时观察到的作用相似；然而，病毒糖蛋白并未刺激DNA合成。用饱和水平血清刺激的静止细胞在暴露于病毒糖蛋白3小时后，己糖摄取几乎没有进一步受到刺激。这种行为表明，该糖蛋白可能作用于一个也是血清作用靶点的系统。
