Civil and Environmental Engineering, South Dakota School of Mines and Technology, Rapid City, SD, 57701, USA.
Department of Civil and Environmental Engineering, Rose-Hulman Institute of Technology, Terre Haute, IN, 47803, USA.
Sci Rep. 2020 Oct 12;10(1):17047. doi: 10.1038/s41598-020-72866-y.
Given our vast lignocellulosic biomass reserves and the difficulty in bioprocessing them without expensive pretreatment and fuel separation steps, the conversion of lignocellulosic biomass directly into electricity would be beneficial. Here we report the previously unexplored capabilities of thermophilic Geobacillus sp. strain WSUCF1 to generate electricity directly from such complex substrates in microbial fuel cells. This process obviates the need for exogenous enzymes and redox mediator supplements. Cyclic voltammetry and chromatography studies revealed the electrochemical signatures of riboflavin molecules that reflect mediated electron transfer capabilities of strain WSUCF1. Proteomics and genomics analysis corroborated that WSUCF1 biofilms uses type-II NADH dehydrogenase and demethylmenaquinone methyltransferase to transfer the electrons to conducting anode via the redox active pheromone lipoproteins localized at the cell membrane.
鉴于我们拥有大量的木质纤维素生物质储备，而如果没有昂贵的预处理和燃料分离步骤，生物加工这些生物质将非常困难，因此将木质纤维素生物质直接转化为电能将是有益的。在这里，我们报告了嗜热芽孢杆菌菌株 WSUCF1 的以前未被探索的能力，即在微生物燃料电池中直接从这些复杂的基质中产生电能。该过程避免了对外源酶和氧化还原介体补充剂的需求。循环伏安法和色谱研究揭示了核黄素分子的电化学特征，反映了菌株 WSUCF1 的介导电子转移能力。蛋白质组学和基因组学分析证实，WSUCF1 生物膜使用 II 型 NADH 脱氢酶和去甲基menaquinone 甲基转移酶通过位于细胞膜上的氧化还原活性信息素脂蛋白将电子传递到导电阳极。
