从巴西慢性创伤中分离出的对环丙沙星耐药的吉奥氏库特菌：病例报告。

Ciprofloxacin-Resistant Kerstersia gyiorum Isolated From a Chronic Wound in Brazil: A Case Report.

机构信息

Faculty of Nursing, State University of Rio de Janeiro, Rio de Janeiro, Brazil.

School of Nursing Aurora of Afonso Costa, Fluminense Federal University, Niterói, Brazil.

出版信息

Wound Manag Prev. 2020 Oct;66(10):42-45.

PMID:33048830

Abstract

UNLABELLED

The presence of Kerstersia gyiorum in lower leg wounds has been reported in case studies from several countries.

OBJECTIVE

This study evaluated the antimicrobial susceptibility profile of K gyiorum isolated from a chronic wound.

METHODS

An 85-year-old woman with chronic venous insufficiency presented to an intermediate care unit in Niteroi City, Rio de Janeiro, Brazil, with an instep chronic wound of 14 cm² with wound duration of 6 months. K gyiorum was identified by matrix-assisted laser desorption ionization-time of ﬂight, confirmed by 16S rRNA partial sequence analysis, and classified as resistant for ciprofloxacin by reagent strips(minimum inhibitory concentration [MIC] = 32 µg/mL) and the broth macrodilution method (MIC = 8 µg/mL). Intermediate resistance for ciprofloxacin was verified by microscan (MIC = 2 µg/mL).

CONCLUSION

The authors identified the first, to their knowledge, lower leg wound with K gyiorum in Brazil and verified that it was ciprofloxacin resistant.

摘要

未加标签

从多个国家的病例研究中报告了小腿伤口中存在 Kerstersia gyiorum。

目的

本研究评估了从慢性伤口分离出的 K gyiorum 的抗菌药物敏感性谱。

方法

一位 85 岁的女性因慢性静脉功能不全，在巴西里约热内卢尼泰罗伊市的一个中级护理病房就诊，她的脚背慢性伤口为 14cm²，伤口持续时间为 6 个月。通过基质辅助激光解吸电离飞行时间（matrix-assisted laser desorption ionization-time of flight，MALDI-TOF）鉴定出 K gyiorum，通过 16S rRNA 部分序列分析确认，并通过试剂条（最低抑菌浓度 [minimum inhibitory concentration，MIC] = 32μg/mL）和肉汤微量稀释法（MIC = 8μg/mL）将其分类为对环丙沙星耐药。通过微扫描（MIC = 2μg/mL）验证了对环丙沙星的中介耐药性。