CHIR99021和重组人白细胞介素6促进猪孤雌胚胎发育和囊胚质量。
CHIR99021 and rpIL6 promote porcine parthenogenetic embryo development and blastocyst quality.
作者信息
Yin Shu-Yuan, Sun Bing-Min, Xu Tian, Liu Xin, Huo Li-Jun, Zhang Xia, Zhou Jilong, Miao Yi-Liang
机构信息
Institute of Stem Cell and Regenerative Biology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction (Huazhong Agricultural University), Ministry of Education, Wuhan, Hubei, 430070, China.
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction (Huazhong Agricultural University), Ministry of Education, Wuhan, Hubei, 430070, China.
出版信息
Theriogenology. 2020 Dec;158:470-476. doi: 10.1016/j.theriogenology.2020.08.007. Epub 2020 Aug 17.
Signaling pathways and transcription factors are involved in porcine embryonic development. Here, we demonstrate that glycogen synthase kinase-3 (GSK3) inhibitor, CHIR99021 and recombinant porcine interleukin-6 (rpIL6) significantly promote porcine parthenogenetic blastocyst formation (49.23 ± 8.40% vs 32.34 ± 4.15%), with increased inner cell mass (ICM) cell numbers (7.72 ± 2.30 vs 4.28 ± 1.60) and higher expression of pluripotent genes, such as OCT4, SOX2 and NANOG. Furthermore, CHIR99021 and rpIL6 improve blastocyst quality with increased blastocyst hatching percentage (16.19 ± 1.96% vs 10.25 ± 1.12%) and subsequently porcine pluripotent stem cells (pPSCs) derivation efficiency. These results advance the understanding of porcine pre-implantation development and provide evidences in improving the blastocyst quality.
信号通路和转录因子参与猪的胚胎发育。在此，我们证明糖原合酶激酶-3（GSK3）抑制剂CHIR99021和重组猪白细胞介素-6（rpIL6）显著促进猪孤雌胚泡形成（49.23±8.40%对32.34±4.15%），同时内细胞团（ICM）细胞数量增加（7.72±2.30对4.28±1.60），多能基因如OCT4、SOX2和NANOG的表达更高。此外，CHIR99021和rpIL6提高了胚泡质量，胚泡孵化率增加（16.19±1.96%对10.25±1.12%），随后提高了猪多能干细胞（pPSCs）的衍生效率。这些结果推进了对猪植入前发育的理解，并为提高胚泡质量提供了证据。
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