Canalis E, McCarthy T, Centrella M
Endocrinology. 1987 Sep;121(3):1198-200. doi: 10.1210/endo-121-3-1198.
Rat calvariae are known to secrete a bone-derived growth factor (BDGF) that stimulates bone DNA and collagen synthesis. BDGF was purified from calvarial culture medium with dialysis, gel filtration and HPLC. Amino acid composition of BDGF was compatible with murine beta 2 microglobulin (beta 2 m), and amino terminal sequence analysis revealed identity with mature murine beta 2 m. This was confirmed by Western blot analysis using a polyclonal antibody to beta 2 m. Like BDGF, human beta 2 m stimulated bone DNA, collagen and noncollagen protein synthesis. Thus, BDGF, an autologous regulator of bone formation, is homologous to beta 2 m.
已知大鼠颅骨可分泌一种骨源性生长因子（BDGF），该因子能刺激骨DNA和胶原蛋白的合成。通过透析、凝胶过滤和高效液相色谱法从颅骨培养基中纯化出BDGF。BDGF的氨基酸组成与小鼠β2微球蛋白（β2m）相符，氨基末端序列分析显示其与成熟的小鼠β2m相同。使用针对β2m的多克隆抗体进行的蛋白质印迹分析证实了这一点。与BDGF一样，人β2m也能刺激骨DNA、胶原蛋白和非胶原蛋白的合成。因此，作为骨形成的自体调节因子，BDGF与β2m同源。
