College of Food Science, South China Agricultural University, Guangzhou 510642, China.
College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Carbohydr Polym. 2020 Dec 15;250:116958. doi: 10.1016/j.carbpol.2020.116958. Epub 2020 Aug 20.
A water-soluble polysaccharide named CYP-1 was isolated from Chinese yam. CYP-1 was characterized as a mannoglucan having a backbone consisting predominately of 1,4-α-linked Glcp branched at O-2, O-3, and O-6 position by t-α-linked Manp with a molecular weight of 2.86 kDa. CYP-1 could inhibit the overproduction of pro-inflammatory cytokines (such as TNF-α and IL-1β) in LPS-induced RAW 264.7 cells and DSS-induced colitis mice. Oral administration of CYP-1 dramatically alleviated colonic pathological damage, suppressed the activation of colonic inflammatory signaling pathways (such as NF-κB and NLRP3 inflammasome), recovered the mRNA expression of junctional proteins (such as ZO-1, claudin-1, occludin, and connexin-43), and modulated the gut microbiota by decreasing the abundances of Alistipes, Helicobacter, and an unidentified Enterobacteriaceae, in DSS-induced colitis mice. Overall, the present study elucidated that a new polysaccharide structure CYP-1 from Chinese yam and its therapeutic potential as a prebiotic for the prevention of inflammatory bowel disease.
从山药中分离得到一种水溶性多糖 CYP-1。CYP-1 被表征为甘露葡聚糖，其主链主要由 1,4-α-连接的 Glcp 组成，在 O-2、O-3 和 O-6 位置分支，由 t-α-连接的 Manp 组成，分子量为 2.86 kDa。CYP-1 可以抑制 LPS 诱导的 RAW 264.7 细胞和 DSS 诱导的结肠炎小鼠中促炎细胞因子（如 TNF-α和 IL-1β）的过度产生。CYP-1 的口服给药可显著减轻结肠病理损伤，抑制结肠炎症信号通路（如 NF-κB 和 NLRP3 炎性体）的激活，恢复连接蛋白（如 ZO-1、claudin-1、occludin 和 connexin-43）的 mRNA 表达，并通过降低 DSS 诱导的结肠炎小鼠中 Alistipes、Helicobacter 和一种未鉴定的肠杆菌科的丰度来调节肠道微生物群。总的来说，本研究阐明了山药中一种新的多糖结构 CYP-1 及其作为预防炎症性肠病的益生元的治疗潜力。
