Wei Xuelin, Dai Juan, Liu Ruijia, Wan Guochao, Gu Shiyu, Du Yuwei, Yang Xinyue, Wang Lijun, Huang Yukun, Chen Pengfei, Chen Xianggui, Yang Xiao, Wang Qin
School of Food and Bioengineering, Xihua University, Chengdu 610039, China.
School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China.
Nutrients. 2024 Apr 12;16(8):1145. doi: 10.3390/nu16081145.
Inflammatory bowel disease (IBD) has attracted much attention worldwide due to its prevalence. In this study, the effect of a solid-in-oil-in-water (S/O/W) emulsion with Caffeic acid phenethyl ester (CAPE, a polyphenolic active ingredient in propolis) on dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice was evaluated. The results showed that CAPE-emulsion could significantly alleviate DSS-induced colitis through its effects on colon length, reduction in the disease activity index (DAI), and colon histopathology. The results of ELISA and Western blot analysis showed that CAPE-emulsion can down-regulate the excessive inflammatory cytokines in colon tissue and inhibit the expression of p65 in the NF-κB pathway. Furthermore, CAPE-emulsion promoted short-chain fatty acids production in DSS-induced colitis mice. High-throughput sequencing results revealed that CAPE-emulsion regulates the imbalance of gut microbiota by enhancing diversity, restoring the abundance of beneficial bacteria (such as ), and suppressing the abundance of harmful bacteria (such as , ). The results of fecal metabolome showed that CAPE-emulsion restored the DSS-induced metabolic disorder by affecting metabolic pathways related to inflammation and cholesterol metabolism. These research results provide a scientific basis for the use of CPAE-emulsions for the development of functional foods for treating IBD.
炎症性肠病（IBD）因其患病率在全球范围内备受关注。在本研究中，评估了一种水包油包固体（S/O/W）乳液与咖啡酸苯乙酯（CAPE，蜂胶中的一种多酚活性成分）对葡聚糖硫酸钠（DSS）诱导的C57BL/6小鼠结肠炎的影响。结果表明，CAPE乳液可通过对结肠长度、疾病活动指数（DAI）降低以及结肠组织病理学的影响，显著减轻DSS诱导的结肠炎。ELISA和蛋白质印迹分析结果表明，CAPE乳液可下调结肠组织中过度的炎性细胞因子，并抑制NF-κB通路中p65的表达。此外，CAPE乳液促进了DSS诱导的结肠炎小鼠中短链脂肪酸的产生。高通量测序结果显示，CAPE乳液通过增强多样性、恢复有益菌（如 ）的丰度以及抑制有害菌（如 、 ）的丰度来调节肠道微生物群的失衡。粪便代谢组学结果表明，CAPE乳液通过影响与炎症和胆固醇代谢相关的代谢途径，恢复了DSS诱导的代谢紊乱。这些研究结果为使用CPAE乳液开发治疗IBD的功能性食品提供了科学依据。
