Departamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual Paulista, 130506-900 SP Rio Claro, Brazil.
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands.
Molecules. 2020 Oct 9;25(20):4596. doi: 10.3390/molecules25204596.
subsp. () is an important phytopathogen and causes Asiatic Citrus Canker (ACC). To control ACC, copper sprays are commonly used. As copper is an environmentally damaging heavy metal, new antimicrobials are needed to combat citrus canker. Here, we explored the antimicrobial activity of chalcones, specifically the methoxychalcone BC1 and the hydroxychalcone T9A, against and the model organism . BC1 and T9A prevented growth of and in concentrations varying from 20 µg/mL to 40 µg/mL. BC1 and T9A decreased incorporation of radiolabeled precursors of DNA, RNA, protein, and peptidoglycan in and . Both compounds mildly affected respiratory activity in , but T9A strongly decreased respiratory activity in . In line with that finding, intracellular ATP decreased strongly in upon T9A treatment, whereas BC1 increased intracellular ATP. In , both compounds resulted in a decrease in intracellular ATP. Cell division seems not to be affected in , and, although in the formation of FtsZ-rings is affected, a FtsZ GTPase activity assay suggests that this is an indirect effect. The chalcones studied here represent a sustainable alternative to copper for the control of ACC, and further studies are ongoing to elucidate their precise modes of action.
亚种（）是一种重要的植物病原菌，可引起亚洲柑橘溃疡病（ACC）。为了控制 ACC，通常会使用铜喷雾。由于铜是一种对环境有害的重金属，因此需要新的抗菌剂来对抗柑橘溃疡病。在这里，我们研究了查耳酮的抗菌活性，特别是甲氧基查耳酮 BC1 和羟基查耳酮 T9A，针对 和模式生物 。BC1 和 T9A 在 20 µg/mL 到 40 µg/mL 的浓度范围内阻止了 和 的生长。BC1 和 T9A 减少了 和 中放射性标记前体的掺入。DNA、RNA、蛋白质和肽聚糖。这两种化合物都轻微影响了 的呼吸活性，但 T9A 强烈降低了 的呼吸活性。与此发现一致，T9A 处理后 中的细胞内 ATP 大量减少，而 BC1 则增加了细胞内 ATP。在 中，这两种化合物都导致细胞内 ATP 减少。细胞分裂似乎没有受到影响，虽然在 中 FtsZ-环的形成受到影响，但 FtsZ GTPase 活性测定表明这是一种间接影响。研究中使用的查耳酮代表了控制 ACC 的可持续铜替代品，正在进行进一步的研究以阐明其确切的作用机制。
