Comparison of the effect on proliferation of osteoblasts MC3T3E1 between the concentrated growth factor extract and the plateletrich fibrin extract.

OBJECTIVES

To compare the effect on proliferation of osteoblasts MC3T3-E1 between the concentrated growth factor extract (CGFe) and the platelet-rich fibrin extract (PRFe).

METHODS

CGFe and PRFe were prepared. MC3T3-E1 was cultured in DMEM medium containing CGFe (10%, 20%, or 30%) and PRFe (10%, 20%, or 30%). The proliferation of MC3T3-E1 was detected by MTT assay at Day 1, 3, 5, and 7. ALP activity was detected by alkaline phosphatase (ALP) staining at Day 1, 3, 5, and 7, and mRNA expressions of Runt-related transcription factor 2 (Runx2) and Osterix (Osx) were detected by quantitative RT-PCR (RT-qPCR) at Day 3 and 7.

RESULTS

Compared with the control group, CGFe and PRFe promoted the proliferation of MC3T3-E1 at Day 1, 3, 5, and 7 (all <0.05). Except for the first day, the proliferation activity in the CGFe group was higher than that in the PRFe group (all <0.05). At Day 1, 3, 5, and 7, compared with the control group, the ALP activities in the CGFe group and the PRFe group were significantly increased (all <0.05). Except for the first day, the ALP activity in the CGFe group was higher than that in the PRFe group (all <0.05). At Day 3 and 7, compared with the control group, the mRNA expression levels of Osx and Runx2 in the CGFe group and the PRFe group were significantly increased (all <0.05); compared with PRFe group, the mRNA expression level of Osx in the CGFe group was significantly higher than that in the PRFe group, and the mRNA expression level of Runx2 was significantly lower than that in the PRFe group (all <0.05).

CONCLUSIONS

CGFe could promote the proliferation of MC3T3-E1 stronger than PRFe, which might be related to the increase of ALP activity and up-regulation of Osx expression.