从白色念珠菌中纯化肌动蛋白并与念珠菌48,000道尔顿蛋白进行比较。
Purification of actin from Candida albicans and comparison with the Candida 48,000-Mr protein.
作者信息
Fiss E, Buckley H R
出版信息
Infect Immun. 1987 Sep;55(9):2324-6. doi: 10.1128/iai.55.9.2324-2326.1987.
Abstract
Actin was purified from Candida albicans cells by affinity chromatography by DNase-Sepharose and was recognized by immunoblotting with monoclonal antibody directed against chick muscle actin. The C. albicans 48-kilodalton protein recognized by sera from patients with invasive candidiasis was shown by DEAE chromatography and immunoblotting not to be identical with the purified C. albicans actin.
摘要
通过用脱氧核糖核酸酶-琼脂糖亲和层析从白色念珠菌细胞中纯化肌动蛋白，并用针对鸡肌肉肌动蛋白的单克隆抗体进行免疫印迹识别。通过离子交换层析和免疫印迹表明，侵袭性念珠菌病患者血清识别的白色念珠菌48千道尔顿蛋白与纯化的白色念珠菌肌动蛋白不同。
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