Maret G, Blanot D, Welti M, Fauchere J L
Int J Pept Protein Res. 1987 Jun;29(6):734-8. doi: 10.1111/j.1399-3011.1987.tb02306.x.
Classical methods of peptide synthesis in solution were used for the preparation of the two tetrapeptides alanyl-lysyl-arginyl-tyrosine and acetyl-alanyl-lysyl-arginyl-tyrosine-(N-methylamide). The two compounds were able to be recognized as substrates by proenkephalin processing enzymes and were used for the development of a quantitative assay for these enzymes. The first substrate proved to be convenient, although it was also partially degraded by amino- and carboxypeptidases under the conditions of the assay. The second was found to be hydrolyzed by the endopeptidases at too slow a rate to allow its routine use in the assay.
采用溶液中肽合成的经典方法制备了两种四肽，即丙氨酰-赖氨酰-精氨酰-酪氨酸和乙酰基-丙氨酰-赖氨酰-精氨酰-酪氨酸-（N-甲基酰胺）。这两种化合物能够被脑啡肽原加工酶识别为底物，并被用于开发这些酶的定量测定方法。第一种底物被证明是方便的，尽管在测定条件下它也会被氨基肽酶和羧肽酶部分降解。发现第二种底物被内肽酶水解的速度太慢，无法在测定中常规使用。
