College of Science and Engineering, Flinders University, Adelaide, Australia E-mail:
Water Sci Technol. 2020 Sep;82(6):1062-1069. doi: 10.2166/wst.2020.379.
High rate algal ponds (HRAPs) are shallow, mixed systems for wastewater treatment, which use sunlight exposure for disinfection. Little is known regarding the relationships between the bacteria and viruses within HRAP systems. Uniquely, flow cytometry permits the rapid identification of bacterial and viral populations in wastewater samples, separating populations based on genome and particle size. Treated wastewater samples were collected from an HRAP at Kingston on Murray, South Australia. Flow cytometry analysis detected bacterial populations and discriminated virus-like particles (VLP) and large VLP (LVLP). Rapid, short term, fluctuations in the abundance of all three populations were observed. Changes in the abundance of these populations was compared; wastewater composition was used as metadata for the comparisons. Linear regression determined relationships in abundances between bacteria and LVLP (R 0.2985); LVLP and VLP (R 0.5829) and bacteria and VLP (R 0.5778) all with p-values of <0.001. Bacterial, LVLP and VLP abundance positively correlated with each other, indicating potential microbial interactions. Overall, the results suggest a parasitic relationship was occurring and driving the abundances of bacteria and viruses within the system.
高密度藻类塘（HRAP）是一种浅层混合系统，用于废水处理，利用阳光照射进行消毒。对于 HRAP 系统中细菌和病毒之间的关系知之甚少。流式细胞术具有独特的优势，可快速识别废水样本中的细菌和病毒群体，根据基因组和颗粒大小对群体进行分离。从南澳大利亚默里的金斯顿 HRAP 中采集处理后的废水样本。流式细胞术分析检测到细菌群体，并区分病毒样颗粒（VLP）和大 VLP（LVLP）。观察到所有三个群体的丰度都迅速且短期波动。比较了这些群体丰度的变化；废水成分被用作比较的元数据。线性回归确定了细菌和 LVLP（R 0.2985）之间；LVLP 和 VLP（R 0.5829）以及细菌和 VLP（R 0.5778）之间丰度的关系，所有这些都具有 <0.001 的 p 值。细菌、LVLP 和 VLP 的丰度相互正相关，表明存在潜在的微生物相互作用。总体而言，结果表明系统内的细菌和病毒的丰度受寄生关系的影响。
