通过超微结构放射自显影法对胰岛素分泌颗粒中的糖蛋白进行定位。
Localization of glycoproteins in insulin secretory granules by ultrastructural autoradiography.
作者信息
Haddad A, Kopriwa B, Pelletier G
出版信息
J Histochem Cytochem. 1987 Oct;35(10):1059-62. doi: 10.1177/35.10.3305700.
PMID:3305700
Abstract
To determine whether or not the secretory granules of insulin-secreting cells contained glycoproteins, isolated rat pancreatic islets were incubated for 2 and 4 hr in a medium containing L-[3H]-fucose. Quantitative analysis of high-resolution electron microscopic autoradiographs of the insulin-secreting beta cells demonstrated that glycoproteins with fucose residues are contained within the insulin secretory granule.
摘要
为了确定胰岛素分泌细胞的分泌颗粒是否含有糖蛋白，将分离的大鼠胰岛在含有L-[3H]-岩藻糖的培养基中孵育2小时和4小时。对胰岛素分泌β细胞的高分辨率电子显微镜放射自显影片进行定量分析表明，含有岩藻糖残基的糖蛋白存在于胰岛素分泌颗粒中。
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