Kanoh T, Ohnaka T, Uchino H
J Histochem Cytochem. 1987 Oct;35(10):1157-60. doi: 10.1177/35.10.3305704.
The plasma cell labeling index (LI), in spite of being a reliable indicator for diagnosis and prognosis of multiple myeloma, has been measured in a limited number of laboratories because of technical difficulties. We have developed a new combined technique, using the peroxidase-antiperoxidase (PAP) method and autoradiography, which has several advantages over previously described methods. The primary advantages of our method in the determination of lymphoid-plasma cell LI% are: (a) no damage to slides during storage of more than 1 year; (b) an exact LI measurement in each morphological variety of pleomorphic immunoglobulin-containing cells; (c) no problem in differentiation of lymphoid plasma cells from early red cell precursors; and (d) a separate LI measurement for those lymphoid-plasma cells composed chiefly, if not exclusively, of monoclonal or neoplastic cells. Because of these advantages, this accurate and less difficult technique will facilitate performance of lymphoid plasma cell LI in a number of laboratories.
尽管浆细胞标记指数（LI）是诊断和预测多发性骨髓瘤的可靠指标，但由于技术难题，只有少数实验室能够进行测量。我们开发了一种新的联合技术，即使用过氧化物酶-抗过氧化物酶（PAP）方法和放射自显影术，该技术比之前描述的方法具有多个优势。我们的方法在测定淋巴浆细胞LI%方面的主要优势包括：（a）在保存超过1年的过程中对玻片无损伤；（b）对各种形态的含多形性免疫球蛋白细胞进行精确的LI测量；（c）在区分淋巴浆细胞和早期红细胞前体方面不存在问题；（d）对那些主要由单克隆或肿瘤细胞（如果不是完全由其组成）构成的淋巴浆细胞进行单独的LI测量。由于这些优势，这种准确且难度较小的技术将有助于多个实验室开展淋巴浆细胞LI的检测。
