从基孔肯雅病毒 SL11131 株构建和鉴定一个感染性克隆。

Construction and characterization of an infectious clone generated from Chikungunya virus SL11131 strain.

机构信息

Department of Microbiology and Infection Control, Osaka Medical College, Takatsuki, Japan.

Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand; Laboratory of Virus-Host Coevolution, Institute for Frontier Life and Medical Sciences, Resilience Research Unit, Kyoto University, Kyoto, Japan.

出版信息

Virology. 2021 Jan 2;552:52-62. doi: 10.1016/j.virol.2020.09.006. Epub 2020 Oct 6.

DOI:10.1016/j.virol.2020.09.006

PMID:33059320

Abstract

Chikungunya virus (CHIKV) is a mosquito-borne RNA virus that causes Chikungunya fever in humans. In this study, we generated two DNA-based CHIKV infectious clones derived from an Indian Ocean Lineage SL11131 strain and a prototype Ross strain. When the replication capabilities of the infectious CHIKV in various cell lines were evaluated, the SL11131 strain was found to replicate more efficiently than the Ross strain in Aedes albopictus C6/36 cells, whereas SL11131 underwent limited replication in a BHK-21-derivative cell line named BHK-DRV. Infection experiments using chimeric CHIKV between SL11131 and Ross revealed that these different replication activities of SL11131 in C6/36 and BHK-DRV cells were determined by structural and nonstructural genes, respectively. Therefore, the infectious clones created in this study will be a useful tool for investigating the virological features of a recent epidemic strain of CHIKV and benefit the development of effective prevention and treatment of CHIKV infection.

摘要

基孔肯雅病毒（CHIKV）是一种由蚊子传播的 RNA 病毒，可引起人类基孔肯雅热。在这项研究中，我们构建了源自印度洋谱系 SL11131 株和原型 Ross 株的两个基于 DNA 的 CHIKV 感染性克隆。当评估传染性 CHIKV 在各种细胞系中的复制能力时，发现 SL11131 株在白纹伊蚊 C6/36 细胞中的复制效率比 Ross 株更高，而 SL11131 在名为 BHK-DRV 的 BHK-21 衍生细胞系中复制受限。使用 SL11131 和 Ross 之间的嵌合 CHIKV 进行感染实验表明，SL11131 在 C6/36 和 BHK-DRV 细胞中的这些不同复制活性分别由结构基因和非结构基因决定。因此，本研究中构建的感染性克隆将成为研究 CHIKV 近期流行株病毒学特征的有用工具，并有助于开发有效的 CHIKV 感染预防和治疗方法。