Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Toxicol Mech Methods. 2021 Feb;31(2):107-115. doi: 10.1080/15376516.2020.1838019. Epub 2020 Nov 8.
Methylglyoxal (MG) is a highly reactive aldehyde able to form covalent adducts with proteins and nucleic acids, disrupting cellular functions. In this study, we performed a screening of () strains to find out which genes of cells are responsive to MG, emphasizing genes against oxidative stress and DNA repair. Yeast strains were grown in the YPD-Galactose medium containing MG (0.5 to 12 mM). The tolerance to MG was evaluated by determining cellular growth and cell viability. The toxicity of MG was more pronounced in the strains with deletion in genes engaged with DNA repair checkpoint proteins, namely Rad23 and Rad50. MG also impaired the growth and viability of mutant strains Glo1 and Gsh1, both components of the glyoxalase I system. Differently, the strains with deletion in genes encoding for antioxidant enzymes were apparently resistant to MG. In summary, our data indicate that DNA repair and MG detoxification pathways are keys in the control of MG toxicity in
甲基乙二醛 (MG) 是一种具有高反应性的醛，能够与蛋白质和核酸形成共价加合物，破坏细胞功能。在这项研究中，我们对 ( ) 株进行了筛选，以找出细胞中对 MG 有反应的基因，强调对抗氧化应激和 DNA 修复的基因。酵母菌株在含有 MG（0.5 至 12 mM）的 YPD-半乳糖培养基中生长。通过测定细胞生长和细胞活力来评估 MG 的耐受性。在缺失与 DNA 修复检查点蛋白（即 Rad23 和 Rad50）相关的基因的菌株中，MG 的毒性更为明显。MG 还损害了 Glo1 和 Gsh1 这两个糖氧酶 I 系统组成部分的 突变菌株的生长和活力。相反，编码抗氧化酶的基因缺失的菌株显然对 MG 有抗性。总之，我们的数据表明，DNA 修复和 MG 解毒途径是控制 MG 毒性的关键。
