Fu Xue, Yang Yang, Shen Yan-Qing, Zhou Xiao-Guang, Zhou Xiao-Yu
Neonatal Medical Center, Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2020 Oct;22(10):1125-1130. doi: 10.7499/j.issn.1008-8830.2004071.
To study the continuous expression and potential function of circular RNA (circRNA), circ4:150439343|150477468 and circ15:73330849|73343359, in mouse lung development.
According to the stage of lung development, lung tissue samples were collected from mice on embryonic day 16.5 (E16.5), embryonic day 18.5 (E18.5), and postnatal day 2 (P2). Hematoxylin and eosin staining was performed to observe the morphology of lung tissue. Quantitative real-time PCR (qRT-PCR) was used to measure the mRNA expression of circ4:150439343|150477468 and circ15:73330849|73343359 during late lung development; miRanda and TargetScan were used to predict the target miRNAs of circRNAs, and then GO and KEGG analysis was performed for the target genes to predict the potential function of circRNAs.
Type II alveolar epithelial cells were observed in the lung slices of E16.5 mice, with a gradual increase in number. On P2, the pulmonary alveoli expanded rapidly, the pulmonary interstitium became thinner, and the alveolar structure gradually became mature. The results of qRT-PCR showed that the relative expression of circ4:150439343|150477468 was continuously upregulated over time and the relative expression of circ15:73330849|73343359 was first downregulated and then upregulated (P<0.05). The KEGG and GO analysis showed that circRNAs were involved in the Notch, PI3K-Akt, and NF-κB signaling pathways.
Circ4:150439343|150477468 and circ15:73330849|73343359 can participate in lung development through the Notch signaling pathway.
研究环状RNA（circRNA）circ4:150439343|150477468和circ15:73330849|73343359在小鼠肺发育过程中的持续表达及潜在功能。
根据肺发育阶段，收集胚胎第16.5天（E16.5）、胚胎第18.5天（E18.5）和出生后第2天（P2）小鼠的肺组织样本。进行苏木精-伊红染色以观察肺组织形态。采用定量实时PCR（qRT-PCR）检测circ4:150439343|150477468和circ15:73330849|73343359在肺发育后期的mRNA表达；利用miRanda和TargetScan预测circRNAs的靶标miRNAs，然后对靶标基因进行GO和KEGG分析以预测circRNAs的潜在功能。
在E16.5小鼠的肺切片中观察到II型肺泡上皮细胞，其数量逐渐增加。在P2时，肺泡迅速扩张，肺间质变薄，肺泡结构逐渐成熟。qRT-PCR结果显示，circ4:150439343|150477468的相对表达随时间持续上调，circ15:73330849|73343359的相对表达先下调后上调（P<0.05）。KEGG和GO分析表明，circRNAs参与Notch、PI3K-Akt和NF-κB信号通路。
Circ4:150439343|150477468和circ15:733308,49|73343359可通过Notch信号通路参与肺发育。
