Smt3, a homologue of yeast SUMO, contributes to asexual development, environmental adaptation, and host infection of a filamentous entomopathogen.

Small ubiquitin-like modifiers (SUMOs) act as the modifiers that regulate several important eukaryotic cell events during sumoylation, but little is known about the functions of SUMO or sumoylation in filamentous entomopathogens. Here, we report the important roles of a single SUMO-encoding gene, smt3, in Beauveria bassiana, a filamentous fungal insect pathogen that serves as a main source of wide-spectrum fungal insecticides. The deletion of smt3 led to significant growth defects on the minimal media with different carbon and nitrogen sources, an obvious reduction (45.7 %) in aerial conidiation during optimal cultivation, and increasing sensitivities to metal ions, oxidation, cell wall perturbation, and the fungicide carbendazim during conidial germination and/or colony growth. Compared with the wild-type, the percentage of germination of conidia stored at 4 °C decreased by 83.9 %, and virulence to Galleria mellonella via normal infection was delayed by 24.6 %. However, conidial thermotolerance increased slightly by 11.4 % in Δsmt3. These findings concurred with the repressed transcripts of some phenotype-related genes and decreased activities of antioxidant enzymes. Taken together, smt3 or sumoylation plays vital roles in the asexual development, environmental adaptation, and pathogenicity of B. bassiana.