Takashima Ippei, Inoue Yohei, Matsumoto Nobuyuki, Takagi Akira, Okuda Kensuke
Laboratory of Bioorganic & Natural Products Chemistry, Kobe Pharmaceutical University, 4-19-1 Motoyama-kita, Higashinada, Kobe 658-8558, Japan.
Chem Commun (Camb). 2020 Nov 11;56(87):13327-13330. doi: 10.1039/d0cc05315e. Epub 2020 Oct 16.
Labile zinc plays various roles in cells at low concentrations which most fluorescent probes are not able to detect. Here we report a cephem-based probe which coordinates to zinc and zinc-bound water cleaves the scaffold and releases the fluorophore. In addition, the zinc is recycled and reacts with multiple probes, amplifying the signal. This signal amplification system is useful for the detection of intracellular zinc at low concentrations and has potential for further development of probes with a similar molecular design.
不稳定锌在细胞中以低浓度发挥着多种作用，而大多数荧光探针无法检测到这些作用。在此，我们报告一种基于头孢菌素的探针，它与锌配位，与锌结合的水会裂解支架并释放荧光团。此外，锌会被循环利用并与多个探针反应，从而放大信号。这种信号放大系统有助于检测低浓度的细胞内锌，并且具有进一步开发具有类似分子设计的探针的潜力。
