[Genotyping of Duffy Blood Group by Microchip Capillary Electrophoresis].

OBJECTIVE

To explore a method for rapidly screening the Duffy blood group genotypes and to establish an information bank of rare blood type donors.

METHODS

The microfluidic capillary electrophoresis system and PCR-SSP method were used to analyze the Duffy genotype of 3 936 unrelated O-type blood donors in our center from December 2014 to September 2018. The serologic identification and typing of other blood type system phenotypes for FY-negative specimen were performed. The Fy (a-b-) specimen was sequenced for genotyping.

RESULTS

The phenotypes of FY-negative specimens were consistent with the genotyping results of the microfluidic capillary electrophoresis system. The results of Duffy phenotyping in 3 936 specimens were follows: Fy (a+b-) 3 564 (90.54%), Fy(a+b+) 353 (8.97%), Fy(a-b+) 18 (0.46%), Fy (a-b-) 1 (0.03%). The gene frequency was FYA (95.03%), FYB (4.94%), and FY*Null (0.03%). The bank of rare blood types of 19 FY-negative specimens was established.

CONCLUSION

Microfluidic capillary electrophoresis system is suitable for Duffy blood group genotyping screening. It can be used to establish a bank of rare blood type, so as to solve the problem of urgent blood transfusion in patients with rare blood type, and to improve blood transfusion safety.