Smal J, Closset J, Hennen G, De Meyts P
Reprod Nutr Dev (1980). 1987;27(2B):521-2.
The 20K variant of native (22K) hGH is a full agonist for the growth promoting and lactogenic properties of the hormone in vivo, but has been reported to have reduced or no insulin-like properties. To explore whether these differences could be explained at the receptor level, we compared the binding of 22K and 20K hGH to receptors in isolated rat adipocytes, a target for the insulin-like effects of the hormone. We compared then the biological insulin-like effects of both hormones using a sensitive assay based on the stimulation of lipogenesis in isolated rat adipocytes. The 20K variant was only 3% as potent as 22K hGH for binding and bioactivity, demonstrating that rat adipocyte receptors are different from those promoting the growth effect.
天然（22K）hGH的20K变体在体内对该激素的促生长和催乳特性而言是一种完全激动剂，但据报道其胰岛素样特性有所降低或不存在。为了探究这些差异是否能在受体水平得到解释，我们比较了22K和20K hGH与分离的大鼠脂肪细胞中受体的结合情况，脂肪细胞是该激素胰岛素样作用的靶标。然后，我们使用基于刺激分离的大鼠脂肪细胞中脂肪生成的灵敏测定法，比较了两种激素的生物胰岛素样作用。20K变体在结合和生物活性方面的效力仅为22K hGH的3%，这表明大鼠脂肪细胞受体与促进生长效应的受体不同。
