Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
College of Animal Science and Technology, Northwest Agriculture & Forestry University, Yangling, China.
Reprod Domest Anim. 2021 Feb;56(2):301-312. doi: 10.1111/rda.13842. Epub 2020 Dec 3.
The purpose of this study was to analyse the effects of season, age, gonad and accessory sex glands on semen characteristics of jackass and to construct multivariate regression models to predict semen quality. In autumn, spring and summer, semen characteristics of 30 sexually mature donkeys (1,014 ejaculations) were analysed to investigate the effect of seasons on semen quality, and gonad and accessory sex gland parameters of 12 jackasses were measured immediately after ejaculation by ultrasonography to investigate the effect of seasons on reproductive organ size. Semen (598 ejaculates), gonad and accessory sex gland parameters of 40 jackasses aged between 3 and 7 years were analysed in autumn to investigate the effects of age and reproductive organ size on semen quality and to construct multivariate models. To verify the accuracy of the models, semen (476 ejaculates), gonad and accessory sex gland parameters of 20 jackasses were measured from March to June. Results revealed that semen, gonad and accessory sex gland parameters were not affected by season and age. Progressive motility (PM) was positively correlated with long axis of the spermatic cord (LASC) and negatively correlated with percentages of sperm abnormality (PSA). Total sperm count (TSC) was positively correlated with testis circumferences (TC) and cross-sectional area of cauda epididymis (CSACE). TC, CSACE, LASC and PSA were included into multivariate models to predict PM, TSC and functional sperm count (FSC) in 20 jackasses (PM = 72.332 + 0.428 LASC - 0.441 PSA; TSC = -169.929 + 8.728 TC + 0.253 CSACE; FSC = -206.645 + 8.788 TC + 0.258 CSACE). The predicted and observed values corresponded well. In conclusion, the tested models can be used for predicting semen quality of donkey.
本研究旨在分析季节、年龄、性腺和附属生殖腺对驴精液特性的影响，并构建多元回归模型来预测精液质量。在秋季、春季和夏季，分析了 30 头性成熟驴（1014 次射精）的精液特性，以研究季节对精液质量的影响；在射精后立即通过超声检查测量了 12 头驴的性腺和附属生殖腺参数，以研究季节对生殖器官大小的影响。在秋季，分析了 40 头 3 至 7 岁的驴的精液（598 次射精）、性腺和附属生殖腺参数，以研究年龄和生殖器官大小对精液质量的影响，并构建多元模型。为了验证模型的准确性，从 3 月至 6 月测量了 20 头驴的精液（476 次射精）、性腺和附属生殖腺参数。结果表明，精液、性腺和附属生殖腺参数不受季节和年龄的影响。前向运动精子（PM）与精索长轴（LASC）呈正相关，与精子畸形率（PSA）呈负相关。总精子数（TSC）与睾丸周长（TC）和附睾尾部横截面积（CSACE）呈正相关。在 20 头驴中，将 TC、CSACE、LASC 和 PSA 纳入多元模型来预测 PM、TSC 和功能性精子数（FSC）（PM=72.332+0.428 LASC-0.441 PSA；TSC=-169.929+8.728 TC+0.253 CSACE；FSC=-206.645+8.788 TC+0.258 CSACE）。预测值与观察值吻合较好。总之，所测试的模型可用于预测驴的精液质量。
