Alani A J, Konrad J, Herbert I V
Res Vet Sci. 1987 Jul;43(1):104-8.
The indirect fluorescent antibody test (IFAT) was used to diagnose some tick-borne infections of sheep, Babesia motasi (Wales), Theileria recondita (Wales) and Cytoecetes phagocytophila. Antigen was prepared from blood derived from splenectomised sheep except for C phagocytophila which was derived from a normal animal. A field survey was made to assess the prevalence of B motasi and T recondita in North Wales and a comparison made between the titres using the B motasi (Wales) antigen with those previously reported. IFA titres reported in the homologous system were consistently lower than those described previously. The results of the field survey suggested that B motasi (Wales) infection is more widespread than was originally thought and more widespread than the known distribution of its vector Haemaphysalis punctata. No serological cross reactions occurred between B motasi (Wales), T recondita (Wales), C phagocytophila, B divergens, Sarcocystis ovicanis and Toxoplasma gondii.
间接荧光抗体试验(IFAT)用于诊断绵羊的一些蜱传感染,如莫氏巴贝斯虫(威尔士株)、隐匿泰勒虫(威尔士株)和嗜吞噬细胞无浆体。抗原除嗜吞噬细胞无浆体来源于正常动物外,其余均取自脾切除绵羊的血液。在北威尔士进行了一项实地调查,以评估莫氏巴贝斯虫和隐匿泰勒虫的流行情况,并比较使用莫氏巴贝斯虫(威尔士株)抗原的滴度与先前报道的滴度。同源系统中报告的间接荧光抗体试验滴度始终低于先前描述的滴度。实地调查结果表明,莫氏巴贝斯虫(威尔士株)感染比最初认为的更为普遍,且比其传播媒介微小牛蜱的已知分布范围更广。莫氏巴贝斯虫(威尔士株)、隐匿泰勒虫(威尔士株)、嗜吞噬细胞无浆体、分歧巴贝斯虫、绵羊肉孢子虫和刚地弓形虫之间未发生血清学交叉反应。