Department of Biology, University of Trnava, Priemyselná 4, 918 43, Trnava, Slovak Republic.
Department of Plant Physiology and Biophysics, Institute of Biological Science, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland.
Plant Physiol Biochem. 2020 Nov;156:591-599. doi: 10.1016/j.plaphy.2020.10.007. Epub 2020 Oct 10.
Eventual protective action of calcium (Ca, 100 or 1000 μM) against cadmium (Cd, 10 or 100 μM) toxicity in common lichen Hypogymnia physodes after 24 h of exposure was studied. Total Cd reached 482 and 2801 μg/g DW in 10 and 100 μM Cd treatments while Ca content reached over 23 mg/g DW in 1000 μM Ca treatment. Ca suppressed Cd accumulation by 23 and 38% in total fraction and completely in absorbed fraction. Fluorescence microscopy of Cd and Ca ions revealed good correlation with quantitative data. Cd stimulated increase in ROS formation and lipid peroxidation as detected using fluorescent reagents and quantification of HO while co-application of Ca suppressed these effects. Formation of nitric oxide was mainly affected by cadmium. Cd depleted amount of amino acids but proteins or phenols remained unaffected by Cd or Ca. On the contrary, sum of thiols, reduced glutathione and ascorbic acid were depleted by Cd but reversed mainly by higher Ca dose. Among organic acids, only Cd-induced depletion of citric acid content was reversed by Ca. Data indicate that ameliorative effect of Ca under Cd excess in lichens is comparable with effect in plants and metabolic responses in various life lineages are discussed.
研究了暴露 24 小时后，钙（Ca，100 或 1000μM）对普通地衣 Hypogymnia physodes 中镉（Cd，10 或 100μM）毒性的最终保护作用。在 10 和 100μM Cd 处理中，总 Cd 含量分别达到 482 和 2801μg/g DW，而在 1000μM Ca 处理中，Ca 含量达到 23 毫克/克 DW 以上。Ca 抑制总分数和吸收分数中 23%和 38%的 Cd 积累。Cd 和 Ca 离子的荧光显微镜与定量数据有很好的相关性。荧光试剂和 HO 的定量检测到 Cd 刺激 ROS 形成和脂质过氧化增加，而 Ca 的共同应用抑制了这些效应。一氧化氮的形成主要受镉的影响。Cd 耗尽了氨基酸的含量，但 Cd 或 Ca 对蛋白质或酚类没有影响。相反，巯基、还原型谷胱甘肽和抗坏血酸的总量被 Cd 耗尽，但主要被更高剂量的 Ca 逆转。在有机酸中，只有 Cd 诱导的柠檬酸含量减少被 Ca 逆转。数据表明，在藻类中，Cd 过量下 Ca 的改善作用与植物中的作用相当，讨论了各种生命谱系中的代谢反应。
