Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei, 230031, Anhui Province, China; Key Laboratory of Genetic Improvement and Ecophysiology of Horticultural Crops, Hefei, 230031, Anhui Province, China.
Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei, 230031, Anhui Province, China; School of Life Sciences, Anhui Agricultural University, Hefei, 230036, Anhui Province, China.
Plant Physiol Biochem. 2020 Nov;156:611-626. doi: 10.1016/j.plaphy.2020.10.003. Epub 2020 Oct 9.
Improving nutrient absorption in pepper has become a vital prerequisite for growth to produce a sustainable yield. In this study, transcriptome gene expression in pepper inoculated with two types of phosphate-solubilizing bacteria (PSB) and grown under low and high nutrient levels (LN and HN) was analyzed. Results showed that the root length increased when pepper was grown under LN; however, the root structure was intensively tight under HN. Our data revealed that the roots preferred horizontal growth than longitudinal growth under HN. PSB strains 'M01' and 'N3' significantly (P < 0.01) increased the P uptake by 70.44% and 98.20%, respectively, but decreased the Ca content by 8.96% and 9.13%, respectively, compared with the control (L1). Although no remarkable difference was detected in the chlorophyll content, inoculation with the two PSB strains decreased the Fe content in pepper under HN. The total clean sequenced data from samples ranged between 5,923,659,118 and 9,955,045,953 bp. Transcriptome profiling revealed 320 upregulated and 449 downregulated genes in L3 versus L1 and 468 upregulated and 532 downregulated genes in L4 versus L1. Gene ontology analysis revealed that the biological processes, including response to stress and secondary metabolic process, were involved. Several pathways were subordinate to glycosphingolipid biosynthesis and linoleic acid and nitrogen metabolisms. Analysis of the eukaryotic orthologous group function revealed that most differential genes were attributed to RNA processing and modification, transcription, and signal transduction. Our results provided new insights into the molecular mechanism related to nutrient uptake in pepper inoculated with PSBs.
提高辣椒的养分吸收能力已成为实现可持续产量的重要前提。本研究分析了接种两种解磷菌（PSB）的辣椒在低氮和高氮养分水平（LN 和 HN）下的转录组基因表达。结果表明，LN 下辣椒根长增加，但 HN 下根结构紧密。我们的数据表明，HN 下根更喜欢水平生长而不是纵向生长。PSB 菌株 'M01' 和 'N3' 分别显著（P < 0.01）提高了 70.44%和 98.20%的磷吸收量，但与对照（L1）相比，分别降低了 8.96%和 9.13%的钙含量。尽管叶绿素含量没有显著差异，但接种两种 PSB 菌株会降低 HN 下辣椒中的铁含量。样本的总清洁测序数据范围在 5923659118 和 9955045933 个碱基对之间。转录组分析显示，与 L1 相比，L3 中有 320 个上调基因和 449 个下调基因，L4 中有 468 个上调基因和 532 个下调基因。基因本体论分析显示，包括应对胁迫和次生代谢过程在内的生物过程参与其中。几个途径隶属于糖脂生物合成和亚油酸和氮代谢。真核同源基因功能分析显示，大多数差异基因与 RNA 加工和修饰、转录和信号转导有关。我们的研究结果为接种 PSB 的辣椒养分吸收相关的分子机制提供了新的见解。
