Institute of Vegetables, Anhui Academy of Agricultural Sciences, Nongke South Road 40, Hefei, 230031, Anhui Province, China.
Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-Construction By Ministry and Province), Hefei, 230031, Anhui Province, China.
Environ Sci Pollut Res Int. 2024 Aug;31(38):50513-50528. doi: 10.1007/s11356-024-34601-2. Epub 2024 Aug 3.
Cadmium (Cd) is a harmful metal in soil, and reducing Cd accumulation in plants has become a vital prerequisite for maintaining food safety. Phosphate-solubilizing bacteria (PSB) can not only improve plant growth but also inhibit the transportation of metals to roots. However, data on gene expression in PSB Burkholderia sp. strain 'N3' and grafted watermelon plants dealing with Cd remain to be elucidated. In this study, core genes and metabolic pathways of strain 'N3' and grafted plants were analyzed by Illumina sequencing. Results showed that 356 and 2527 genes were upregulated in 'N3' and grafted watermelon plants, respectively, whereas 514 and 1540 genes were downregulated in 'N3' and grafted watermelon plants, respectively. Gene ontology enrichment analysis showed that signal transduction, inorganic ion transport, cell motility, amino acid transport, and metabolism pathways were marked in 'N3'. However, pathways such as secondary metabolite biosynthesis, oxidation-reduction process, electron transfer activity, and channel regulator activity were marked in the grafted plants. Six genes related to pentose phosphate, glycolysis, and gluconeogenesis metabolism were upregulated in the grafted plants. This study paves the way for developing potential strategies to improve plant growth under Cd toxicity.
镉（Cd）是土壤中的有害金属，降低植物对镉的积累已成为保障食品安全的重要前提。解磷细菌（PSB）不仅可以促进植物生长，还可以抑制金属向根部的运输。然而，关于 PSB 伯克霍尔德氏菌菌株 'N3' 和嫁接西瓜植株应对镉的基因表达数据仍有待阐明。在本研究中，通过 Illumina 测序分析了菌株 'N3' 和嫁接西瓜植株的核心基因和代谢途径。结果表明，菌株 'N3' 和嫁接西瓜植株分别有 356 个和 2527 个基因上调，而菌株 'N3' 和嫁接西瓜植株分别有 514 个和 1540 个基因下调。基因本体论富集分析表明，信号转导、无机离子运输、细胞运动、氨基酸运输和代谢途径在菌株 'N3' 中显著富集。然而，在嫁接植株中，次生代谢物生物合成、氧化还原过程、电子传递活性和通道调节活性等途径显著富集。与戊糖磷酸、糖酵解和糖异生代谢相关的 6 个基因在嫁接植株中上调。本研究为开发提高植物在镉毒性下生长的潜在策略奠定了基础。
