[G蛋白偶联受体30在染料木黄酮对人甲状腺鳞状细胞SW579凋亡及细胞周期影响中的作用]
[Roles of G protein-coupled receptor 30 in the effects of genistein on apoptosis and cell cycle in human thyroid squamous cells SW579].
作者信息
Chen Zhiqing, Xuan Qun, Zhao Dan, Wu Shaoxiong, Yin Jianzhong, Pan Hongmei
机构信息
School of Public Health, Kunming Medical University, Kunming 650500, China.
School of Basic Medical Sciences, Kunming Medical University, Kunming 650500, China.
出版信息
Wei Sheng Yan Jiu. 2020 Sep;49(5):780-784. doi: 10.19813/j.cnki.weishengyanjiu.2020.05.015.
OBJECTIVE
Investigate the estrogen receptor expression in human thyroid squamous cell carcinoma SW579 and the effects of genistein on the apoptosis and cycle of SW579 and its mechnism.
METHODS
The real-time PCR was applied to detect the expression of estrogen receptor(ER)α、ERβ and G protein-coupled receptor(GPR)30 in human thyroid squamous cell line SW579; MTT was used to test the effect of genistein on cell proliferation in the SW579 cells before and after blocking GPR30; flow cytometry was explorited to measure the effect of genistein on the cell cycle and apoptosis in the SW579 was detected before and after blocking GPR30.
RESULTS
The high concentration of genistein promoted the expression of ERβ and GPR30 in the SW579 cells, but ERα was not expressed. The specific blocking of GPR30, the cell proliferation was aboviously inhibited by genistein in the SW579 cells and the cell apoptosis was markedly promoted after the GPR30 was blocked; The cell cycle was mainly blocked in G_2/M phase.
CONCLUSION
Genistein can obiviously promote the cell proliferation in the SW579 cells, which may be related to the action of GPR30.
目的
研究雌激素受体在人甲状腺鳞状细胞癌SW579中的表达，以及染料木黄酮对SW579细胞凋亡、周期的影响及其机制。
方法
应用实时荧光定量PCR检测人甲状腺鳞状细胞系SW579中雌激素受体（ER）α、ERβ及G蛋白偶联受体（GPR）30的表达；采用MTT法检测阻断GPR30前后染料木黄酮对SW579细胞增殖的影响；运用流式细胞术检测阻断GPR30前后染料木黄酮对SW579细胞周期及凋亡的影响。
结果
高浓度染料木黄酮促进SW579细胞中ERβ及GPR30的表达，但未检测到ERα的表达。特异性阻断GPR30后，染料木黄酮对SW579细胞增殖有明显抑制作用，且GPR30阻断后细胞凋亡明显增加；细胞周期主要阻滞于G2/M期。
结论
染料木黄酮可明显促进SW579细胞增殖，这可能与GPR30的作用有关。
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