Qu X, Zhang L
ENT and Maxillofacial Oncology, Tianjin Medical University Cancer Hospital, Tianjin, China.
Genet Mol Res. 2015 Nov 23;14(4):14680-9. doi: 10.4238/2015.November.18.32.
We investigated the effect of an ABCE1-silencing gene on the proliferation, invasion, and migration of human thyroid carcinoma SW579 cells. We designed and synthesized targeted ABCE1-siRNA sequences and a negative control sequence (NC-siRNA), and transfected them into human thyroid cancer SW579 cells by electrotransfer to obtain ABCE1-SW579 and NC-siRNA-SW579 cells (siRNA is small interfering RNA). Through reverse transcription polymerase chain reaction and western blotting analysis, ABCE1 mRNA and protein expression levels in the electrotransferred cells were detected, and flow cytometry was used to detect cell cycle and apoptosis. The cell counting kit-8 (CCK-8) proliferation assay, the scratch healing assay, and the cell invasion assay were used to measure cell proliferation, migration, and invasion capabilities, respectively. Compared with NC-siRNA-SW579 and Ctrl-SW579 groups, ABCE1 mRNA and protein expression levels in the ABCE1-SW579 cells were significantly reduced. The growth rate of ABCE1-SW579 cells was significantly inhibited, the cell cycle was arrested in the G0/G1 phase, and the number of cells in the S phase was reduced. Compared with the Ctrl-SW579 group, the cell apoptosis rate in the ABCE1-SW579 group was significantly higher (P < 0.01), and proliferation, migration, and invasion were significantly reduced (P < 0.05). Expression of the ABCE1-silencing gene, transfected by electrotransfer, could inhibit the proliferation, invasion, and migration of thyroid cancer cells.
我们研究了ABCE1沉默基因对人甲状腺癌SW579细胞增殖、侵袭和迁移的影响。我们设计并合成了靶向ABCE1的小干扰RNA（siRNA）序列和阴性对照序列（NC-siRNA），通过电转染将它们导入人甲状腺癌SW579细胞，以获得ABCE1-SW579和NC-siRNA-SW579细胞（siRNA即小干扰RNA）。通过逆转录聚合酶链反应和蛋白质免疫印迹分析，检测电转染细胞中ABCE1的信使核糖核酸（mRNA）和蛋白表达水平，并使用流式细胞术检测细胞周期和凋亡情况。分别采用细胞计数试剂盒-8（CCK-8）增殖实验、划痕愈合实验和细胞侵袭实验来测定细胞的增殖、迁移和侵袭能力。与NC-siRNA-SW579组和对照-SW579组相比，ABCE1-SW579细胞中ABCE1的mRNA和蛋白表达水平显著降低。ABCE1-SW579细胞的生长速率显著受到抑制，细胞周期停滞在G0/G1期，S期细胞数量减少。与对照-SW579组相比，ABCE1-SW579组的细胞凋亡率显著更高（P<0.01），增殖、迁移和侵袭能力显著降低（P<0.05）。通过电转染导入的ABCE1沉默基因的表达可抑制甲状腺癌细胞的增殖、侵袭和迁移。
