Department of Chemistry, Chungnam National University, Daejeon, Republic of Korea.
Center for Scientific Instrumentation, Korea Basic Science Institute, Daejeon, Republic of Korea.
Food Chem. 2021 Apr 16;342:128356. doi: 10.1016/j.foodchem.2020.128356. Epub 2020 Oct 12.
Disaccharides are sugars composed of two monosaccharides joined by a glycosidic linkage. The specific properties of a disaccharide depend on the type of the glycosidic linkage and the identity of the two component monosaccharides. In this work, seven disaccharide isomers (gentiobiose, isomaltose, melibiose, lactose, maltose, cellobiose, and sucrose) were analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using a graphene oxide matrix. Each disaccharide was identified by its unique cleavage pattern. To determine the feasibility of quantitative analyses based on specific fragment patterns, mixtures of sucrose with cellobiose or maltose were prepared at different ratios and analyzed by MALDI-MS, where a strong linear correlation was observed between the relative peak intensity of the sucrose fragment peak at m/z 185 and the amount of sucrose in the mixture. The calibration curve was successfully applied to obtain the relative amount of maltose and sucrose in four different honey samples.
二糖由通过糖苷键连接的两个单糖组成。二糖的特定性质取决于糖苷键的类型和两个组成单糖的身份。在这项工作中，七种二糖异构体（龙胆二糖、异麦芽糖、蜜二糖、乳糖、麦芽糖、纤维二糖和蔗糖）使用氧化石墨烯基质通过基质辅助激光解吸/电离质谱（MALDI-MS）进行了分析。每种二糖都通过其独特的裂解模式进行鉴定。为了确定基于特定片段模式的定量分析的可行性，将蔗糖与纤维二糖或麦芽糖以不同的比例混合，并通过 MALDI-MS 进行分析，在其中观察到蔗糖碎片峰 m/z 185 的相对峰强度与混合物中蔗糖的量之间存在很强的线性相关性。校准曲线成功地应用于获得四种不同蜂蜜样品中麦芽糖和蔗糖的相对含量。
