Umehara Takashi, Urabe Nao, Obata Toshiki, Yamaguchi Takashi, Tanaka Atsushi, Shimada Masayuki
Graduate School of Integrated Sciences for Life Hiroshima University Higashi-Hiroshima Japan.
Saint Mother Obstetrics and Gynecology Clinic Institute for ART Fukuoka Japan.
Reprod Med Biol. 2020 Aug 26;19(4):415-424. doi: 10.1002/rmb2.12345. eCollection 2020 Oct.
Ovarian vascular abnormality and ovarian fibrosis are observed in the low responder patients and aging mice. Vascularization and fibrosis are regulated by injury-repair system, such as wound. Thus, in this study, the authors tried to investigate the effect of the surgical treatment to ovarian surface with cutting on the functions of ovary in aging mouse model, gcKO.
The ovarian surface of gcKO was surgically cut, and then the ovary was returned inside of bursa ovarica. To assess the effect of cutting on fertility, mating test, smear analysis, and exogenous hormonal treatment were done. Additionally, the histological analysis was used for observing the remodeling of ovarian stroma after the surgical approach.
Ovarian fibrosis disappeared at 7 days after surgery. With the abrogation of fibrosis, the blood vessels were fluently observed around the follicles, and the follicular development was re-started. The responses against exogenous hormone were recovered at 21 days after the surgery, and estrous cycle and delivery were also recovered by the surgery and the fertility was maintained for 3 months.
This cutting method of ovarian surface becomes a good option against low responder patients.
在低反应患者和衰老小鼠中观察到卵巢血管异常和卵巢纤维化。血管生成和纤维化受损伤修复系统（如伤口）调节。因此，在本研究中，作者试图在衰老小鼠模型（gcKO）中研究卵巢表面切割手术治疗对卵巢功能的影响。
对gcKO小鼠的卵巢表面进行手术切割，然后将卵巢放回卵巢囊内。为评估切割对生育能力的影响，进行了交配试验、涂片分析和外源性激素治疗。此外，组织学分析用于观察手术处理后卵巢基质的重塑。
术后7天卵巢纤维化消失。随着纤维化的消除，在卵泡周围可清晰观察到血管，卵泡发育重新开始。术后21天对外源性激素的反应恢复，手术使发情周期和分娩也恢复，生育能力维持了3个月。
这种卵巢表面切割方法成为低反应患者的一个良好选择。
