Zhao Linlin, Liu Xiaoguang, Zhang Jing, Dong Gaoyang, Xiao Weihua, Xu Xin
School of Kinesiology, Shanghai University of Sport, Shanghai, China.
Front Physiol. 2020 Sep 18;11:533690. doi: 10.3389/fphys.2020.533690. eCollection 2020.
The purpose of this study was to investigate the effect of exogenous hydrogen sulfide (HS) treatment on skeletal muscle contusion. We established a skeletal muscle contusion model (S group) and an HS treated of skeletal muscle contusion model (HS group). Gastrocnemius muscles (GMs) were collected at day 1, day 5, day 10, and day 15 after injury, and comprehensive morphological and genetic analyses was conducted. HS treatment reduced M1 macrophage (CD68), profibrotic cytokines (TGF-β), pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6), chemokines (CCL2, CCR2, CCL3, CCL5, CXCL12, and CXCR4), matrix metalloproteinases (MMP-1, MMP-2, MMP-9, and MMP-14) and oxidative stress factor (gp91phox) expression levels, improved M2 macrophage (CD206) level. Thus, exogenous HS treatment reduced inflammation and oxidative stress, attenuated skeletal muscle fibrosis, and partly improved skeletal muscle injury.
本研究的目的是探讨外源性硫化氢（HS）治疗对骨骼肌挫伤的影响。我们建立了骨骼肌挫伤模型（S组）和HS治疗的骨骼肌挫伤模型（HS组）。在损伤后第1天、第5天、第10天和第15天收集腓肠肌，并进行全面的形态学和基因分析。HS治疗降低了M1巨噬细胞（CD68）、促纤维化细胞因子（TGF-β）、促炎细胞因子（TNF-α、IFN-γ、IL-1β和IL-6）、趋化因子（CCL2、CCR2、CCL3、CCL5、CXCL12和CXCR4）、基质金属蛋白酶（MMP-1、MMP-2、MMP-9和MMP-14）以及氧化应激因子（gp91phox）的表达水平，提高了M2巨噬细胞（CD206）水平。因此，外源性HS治疗减轻了炎症和氧化应激，减轻了骨骼肌纤维化，并部分改善了骨骼肌损伤。
