Seger G, Achatz M, Heinze W, Sinsel F
J Histochem Cytochem. 1977 Jul;25(7):707-18. doi: 10.1177/25.7.330724.
A new technique for the fast and quantitative extraction of cell parameters from diffraction patterns was applied to cervical gynecologic material. Based on radial scans through the diffraction pattern, this technique permits the simultaneous determination of the nuclear and cytoplasmic diameter by Fourier analysis of the radial scanning signals after compensation for the intensity falloff by an amplification which is proportional to the third power of the radial position in the diffraction plane. In addition, a correlation between the angle of the highest amplitude of the scanning signals and the coarseness of microstructures in the cell was observed. Illustrative examples of measurements on exfoliated cells of different type are presented.
一种从衍射图样中快速定量提取细胞参数的新技术被应用于宫颈妇科材料。基于对衍射图样的径向扫描，该技术通过对径向扫描信号进行傅里叶分析，在对强度衰减进行补偿后，通过与衍射平面中径向位置的三次方成正比的放大来同时确定细胞核和细胞质的直径。此外，还观察到扫描信号最高振幅角度与细胞内微观结构粗糙度之间的相关性。给出了对不同类型脱落细胞测量的示例。
