Skogen-Hagenson M J, Salzman G C, Mullaney P F, Brockman W H
J Histochem Cytochem. 1977 Jul;25(7):784-9. doi: 10.1177/25.7.330729.
A flow chamber has been developed which collects about 60% of the total cell fluorescence for analysis compared to about 2.5% for conventional flow systems. The chamber, an ellipsoid of revolution, is gold-plated for increased reflectivity. Fluorochrome-stained cells enter the flow cell directly above the primary focus of the ellipsoid at the rate of 1000 cell/sec. A focused argon-ion laser beam enters the flow cell parallel to the semiminor axis and intersects the cell stream at the primary focus. Fluorescent light emanating from this point is reflected toward the secondary focus, where it exits the chamber for analysis. The high efficiency flow cytometer has been used to obtain nucleotide fluorescence distributions from samples of Micrococcus glutamicus bacteria stained with propidium iodide and of spermatozoa stained by the acriflavine-Feulgen procedure.
已开发出一种流动腔室，与传统流动系统约2.5%的细胞荧光收集率相比，该腔室可收集约60%的总细胞荧光用于分析。该腔室为旋转椭球体，镀有金以提高反射率。经荧光染料染色的细胞以每秒1000个细胞的速率直接进入位于椭球体主焦点上方的流动池。一束聚焦的氩离子激光束平行于短半轴进入流动池，并在主焦点处与细胞流相交。从该点发出的荧光被反射向次焦点，在那里它离开腔室进行分析。这种高效流式细胞仪已用于获取用碘化丙啶染色的谷氨酸微球菌细菌样本以及用吖啶黄-福尔根法染色的精子的核苷酸荧光分布。
