通过在金纳米颗粒和葫芦[n]脲聚集体内形成精确的等离子体纳米结实现尿酸的定量表面增强拉曼散射检测

Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril.

作者信息

Chio Weng-I Katherine, Davison Gemma, Jones Tabitha, Liu Jia, Parkin Ivan P, Lee Tung-Chun

机构信息

Department of Chemistry, University College London (UCL).

Department of Chemistry, University College London (UCL); Institute for Materials Discovery, University College London (UCL);

出版信息

J Vis Exp. 2020 Oct 3(164). doi: 10.3791/61682.

DOI:10.3791/61682

PMID:33074261

Abstract

This work describes a rapid and highly sensitive method for the quantitative detection of an important biomarker, uric acid (UA), via surface-enhanced Raman spectroscopy (SERS) with a low detection limit of ~0.2 μM for multiple characteristic peaks in the fingerprint region, using a modular spectrometer. This biosensing scheme is mediated by the host-guest complexation between a macrocycle, cucurbit[7]uril (CB7), and UA, and the subsequent formation of precise plasmonic nanojunctions within the self-assembled Au NP: CB7 nanoaggregates. A facile Au NP synthesis of desirable sizes for SERS substrates has also been performed based on the classical citrate-reduction approach with an option to be facilitated using a lab-built automated synthesizer. This protocol can be readily extended to multiplexed detection of biomarkers in body fluids for clinical applications.

摘要

这项工作描述了一种通过表面增强拉曼光谱（SERS）定量检测重要生物标志物尿酸（UA）的快速且高度灵敏的方法。使用模块化光谱仪，指纹区域多个特征峰的检测限低至约0.2 μM。这种生物传感方案由大环化合物葫芦[7]脲（CB7）与UA之间的主客体络合介导，随后在自组装的金纳米颗粒：CB7纳米聚集体中形成精确的等离子体纳米结。还基于经典的柠檬酸盐还原方法进行了适合SERS底物的理想尺寸金纳米颗粒的简便合成，并可选择使用实验室自制的自动合成仪来促进合成。该方案可轻松扩展至用于临床应用的体液中生物标志物的多重检测。